harassment and discrimination in workplace

Hi Team,

I really need you suggestion and help regarding my issue. My manager at work is harrasing me.

I  asked to leave on raksha bandhan.  she denied the request

I  asked to leave on Diwali.  she denied the request

I asked for leave because my wife was not well-  she denied the request

I asked for leave because I was not well-  she denied the request

I took above leave because of the urgency and I was refer to HR. 

Now she is calculating my performace differently from the other team members and settin different targets for me. My company HR is also with them. What shall I do in this case as I am mentally disturbed. My manager says that Plan leave are subject to availability and can be cancelled anytime. She is very rude with me as well.